Pulsed electromagnetic field (PEMF) transiently stimulates the rate of mineralization in a 3-dimensional ring culture model of osteogenesis

Paul Benya, Aaron Kavanaugh, Martin Zakarian, Philip Söderlind, Tea Jashashvili, Nianli Zhang, Eric Waldorff, James Ryaby & Fabrizio Billi
Pulsed Electromagnetic Frequencies (PEMF) have shown efficacy in bone repair and yet the optimum characteristics of this modality and its molecular mechanism remain unclear. To determine the effects of timing of PEMF treatment we present a three-dimensional ring culture model of osteogenesis that demonstrates strong de novo generation of collagen and mineral matrix and exhibits stimulation by PEMF in multiple stages over 62 days of culture. Mouse postnatal day 2 calvarial pre-osteoblasts were cast within...
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