A manager’s guide to using eDNA metabarcoding in marine ecosystems

Zachary Gold, Adam R. Wall, Teia M. Schweizer, N. Dean Pentcheff, Emily E. Curd, Paul H. Barber, Rachel S. Meyer, Robert Wayne, Kevin Stolzenbach, Kat Prickett, Justin Luedy & Regina Wetzer
Environmental DNA (eDNA) metabarcoding is a powerful tool that can enhance marine ecosystem/biodiversity monitoring programs. Here we outline five important steps managers and researchers should consider when developing eDNA monitoring program: 1) select genes and primers to target focal taxa; 2) assemble or develop comprehensive barcode reference databases; 3) apply rigorous site occupancy based decontamination pipelines; 4) conduct pilot studies to define spatial and temporal variance of eDNA; and 5) archive samples, extracts, and raw...
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