The Use Of Dehydrogenases for Regenerating ß-Nicotinamide Adenine Coenzymes through Discontinuous and Continuous Processes

D.Z. Andreotti, E.J. Tomotani & M. Vitolo
Efficiency is limited when any conversion is carried out with NAD or NADP-dependent dehydrogenase because both the oxidized and reduced forms of the coenzyme are interchanged inside the reactor. Such interchanging was evaluated through batch (conducted for 90min at 50 rpm and 30oC) and continuous (conducted for 30h at 100rpm and 30oC) processes, using a mixture of soluble glucose-6-phosphate dehydrogenase (G6PDH) and glutamate dehydrogenase (GLUDH). The batch and continuous approaches were, respectively, conducted in a...