Immobilization of Cellulase Enzymes on Epoxy Sepabeads® for the Hydrolysis of Lignocellulosic Materials

I. De Bari, A. Verardi, V. Calabrò, F. Liuzzi, E. Ricca & G. Braccio
For several years, international research has been performing the optimization of cellulose enzymatic hydrolysis to produce bioethanol. Enzymes low specific activity and fast deactivation are relevant problems in the development of this process and additional improvements are needed to make bioethanol comparable to traditional fuels. Enzymes immobilization facilitates biocatalyst recovery and reuse. Furthermore, enzymes immobilization avoids autolysis and increase enzyme stability allowing continuous operation for long periods, reducing processes costs and making this process more...