The Use of a Membrane Reactor for the NAD(H) Continuous Regeneration Catalyzed by NAD(H)-dependent Alcohol Dehydrogenase through the Substrate-coupled Procedure

E.J. Tomotani & M. Vitolo
The potential of enzyme­catalyzed transformations is promising for the pharmaceutical and fine­chemical industries. Nonetheless, more complicated enzymatic reactions often require the presence of expensive cofactors (for instance, NAD, NADP). However it requires efficient regeneration of the nicotinamide cofactors to allow for an economically feasible process. The present work focuses on the applicability of alcohol dehydrogenase (ADH) from the yeast Saccharomyces cerevisiae, for regenerating the cofactor continuously using a Bioengineering Enzyme Membrane Reactor®. The process of...