Bio-Conversion of Biodiesel-Derived Glycerol into Hydrogen and Ethanol: Beyond Second-Generation Biofuels

C. Varrone, F. Fiocchetti, B. Giussani, G. Izzo, A. Marone, G. Massini, C. Patriarca, S. Rosa, A. Signorini & A. Wang
Enrichment of activity sludge allowed to select a suitable microbial mixed culture able to grow on crude glycerol as the only carbon source, using a very simple synthetic medium without any extra nutrient supplements. Following to statistical optimisation, the selected pool was able to effectively convert crude glycerol into H2 and ethanol, both at a near¬stoichiometric yield. Subsequently, upscale tests were performed to validate the statistical model, and fed¬batch tests, in non¬sterile conditions, led to...