Optimization of Upstream Processes in Bioethanol Production

J.N. Ntihuga, T. Senn, P. Gschwind & R. Kohlus
Important upstream processes in continuous bio-ethanol fermentation were investigated. Optimum mash concentration, mash particle size and enzyme dosage were studied for liquefaction process. Saccharification process was studied by Double Saccharification Principle (DSP). Total starch analysis kit was used to hydrolyze and quantify the nonsolubilised starch for the liquefaction efficiency estimation. Continuous ethanol fermentation was performed in a Blenke cascade system using substrates from the optimized upstream processes. The results show that the mash with small...