2.2.2 Detection of DNA Sequence Based on Proton Reduction Catalyzed by Deposition of Platinum- Complexes

Tomoyuki Yasukawa, Yuya Yamashita, Daichi Nakayama, Fumio Mizutani & Seiichiro Iijima
A novel electrochemical technique for the detection of DNA sequence has been developed by a simple electrochemical deposition of chloro-2,2':6',2''-terpyridine platinum (II) chloride dihydrate (Ptcomplex) on a glassy carbon electrode. The intercalation of a Pt-complex to a double-stranded DNA (ds-DNA), which is formed with the complementary recognition of probe DNA to target DNA, caused to suppress the electroreduction of the Pt-complex due to the steric hindrance and decrease of the diffusion coefficient. Thus, the catalytic...