2.2.6 Developing Electrochemical Impedance Immunosensor for the Detection of Myoglobin

Naga Siva Kumar Gunda, Susanta Sinha Roy, Sushanta K. Mitra & Minashree Singh
In the present work, electrochemical impedance immunosensor is developed for detection of myoglobin. The procedure involves the immobilization of myoglobin antibodies on the interdigitated gold electrodes (IDE) using alkanethiol self-assembled monolayer (SAM) for binding myoglobin antigens available in the blood serum/aqueous solution. Then the sensor system is characterised using alternating current (AC) electrochemical impedance spectroscopy (EIS), in which the change in impedance spectra is observed across the frequency range of 0.1 Hz to 10 MHz....