3.2.4 [pH]o imaging in proton releasing cells by an ion image sensor-based chemical microscopy

Takashi Sakurai, Hidenori Taki, Koichi Okumura, Kazuaki Sawada & Yasuko Fukushi
To examine the functions of proton release from the cells, we measured the extracellular pH ([pH]o) by using an ion image sensor made of a 2 dimensional array of potential sensitive pixels. We recorded the changes in [pH] o of cells such as osteoclasts, gastric glands and hippocampal slices. After stimulation of cells and/or tissues, [pH]o was decreased, and the degree of the pH changes was dependent on the doses of stimulants. We established a...