P1.2.3 Development of a Love-wave Immunosensor with Microfluidic Technology to Detect Phages in Dynamic Mode

D. Matatagui, M. J. Fernández, J. Fontecha, J. P. Santos, M. C. Horrillo, D. Moynet, J. P. Esquivel, I. Gràcia, C. Cané, C. Déjous & D. Rebière
The goal has been to develop an immunosensor based on a Love-wave device and a PDMS microfluidic chip in order to detect potentially pathogenic microorganisms in real time. Therefore, it has been tested with the phage M13 detected through the mouse monoclonal antibody anti-M13 (AM13). The Double-layer Agar (DLA) technique has been used to quantify the number of phages, linked to the sensor surface, in plaques-forming unit (pfu); that has allowed us to compare the...