P2.1.12 Label Free Biochemical Determinations Based on the Contrast Monitoring of Periodic Patterns

Ioannis Archonta, Alexandros Salapatas & Konstantinos Misiakos
A method is presented for label-free detection of protein-protein binding in real time. It is based on the protein induced contrast modulation of the microscope image of an oxidized and patterned silicon substrate. Interferometric effects make the reflection coefficient of the solution-protein-oxide-silicon stack a function of the oxide-protein combined thickness. As a consequence, the contrast of the pattern changes during the experiment and the magnitude of the change is a measure of the progress of...