B2.4 - Protein Microarrays for Diagnostic Assays

Michael Hartmann, Thomas Joos & Johan Roeraade
A protein microarray, sometimes referred to as a protein chip, allows quantifying a large number of target analytes from minimal samples, using immobilized antibodies as capture molecules. Immobilized proteins in a microarray allow screening for reactive antibodies in serum samples, providing a multiplex approach to identify protein-protein interactions, identifying substrates of protein kinases or identifying the targets of biologically active small molecules. The array is a piece of chemically modified glass on which different capture...