Structural model of the human tRNA multisynthetase complex by cross-linking mass spectrometry

We have taken advantage of cross-linking mass spectrometry (XL-MS) to interrogate the quaternary structure of the human MSC. Using the MS-cleavable cross-linker, DSSO, 19 unique inter-protein cross-links spanning all MSC constituents, and 118 unique intra-protein cross-links, were observed in MSC isolated from human HEK293T cells. The cross-links established spatial relationships between constituent pairs, and between domains in individual constituents. The protein-protein interactions detected by XL-MS were further improved by docking, revealing for the first time...
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