Defining a novel domain which provides an essential contribution to site-specific interaction of Rep protein with DNA

The essential feature of replication initiation proteins is their ability to bind to DNA. In this work, we describe a new domain that contributes to a replication initiator sequence-specific interaction with DNA. Applying biochemical assays and structure prediction methods coupled with DNA-protein cross-linking, mass spectrometry, and construction and analysis of mutant proteins, we identified that the replication initiator of the broad-host-range plasmid RK2, in addition to two winged helix domains, contains a third DNA binding...
This data repository is not currently reporting usage information. For information on how your repository can submit usage information, please see our documentation.