Rumen fluid sampling via oral stomach tubing method

Sanne van Gastelen, Wouter Muizelaar, Paolo Bani, Björn Kuhla, Mogens Larsen & David Yáñez-Ruiz
There are different purposes for collecting rumen fluid, e.g. assessment of rumen fermentation characteristics and rumen microbiota, as well as ex vivo incubations for feed analysis. Collecting rumen content through the fistula from rumen cannulated ruminants is considered the reference method for the collection of representative samples of rumen digesta, but access to surgically-modified animals is not universal and restricted to research facilities. Hence, a less invasive alternative has been developed: the oral stomach tubing...
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