Multivariate Chemometric Assisted Analysis of Metformin Hydrochloride, Gliclazide and Pioglitazone Hydrochloride in Bulk Drug and Dosage Forms

Radhika Bhaskar, Rahul Bhaskar, Mahendra K. Sagar & Vipin Saini
Purpose: In this work a numerical method, based on the use of spectrophotometric data coupled to partial least squares (PLS) regression and net analyte preprocessing combined with classical least square (NAP/CLS) multivariate calibration, is reported for the simultaneous determination of metformin hydrochloride (MET), gliclazide (GLZ) and pioglitazone hydrochloride (PIO) in synthetic samples and combined commercial tablets. Methods: Spectra of MET, GLZ and PIO were recorded at concentrations within their linear ranges (5-25 µg/ml, 0.5-8 µg/ml...