Development and Application of an HPLC Method for Erlotinib Protein Binding Studies

Soheila Bolandnazar, Adeleh Divsalar, Hadi Valizadeh, Arash Khodaei & Parvin Zakeri-Milani
Purpose: The aim of the present study was to develop a simple and rapid reversed-phase high performance liquid chromatographic method with UV detection for erlotinib hydrochloride quantification, which is applicable for protein binding studies. Methods: Ultrafilteration method was used for protein binding study of erlotinib hydrochloride. For sample analysis a simple and rapid reversed-phase high performance liquid chromatographic method with UV detection at 332 nm was developed. The mobile phase was a mixture of methanol,...