A Unique Report: Development of Super Anti-Human IgG Monoclone with Optical Density Over Than 3

Leili Aghebati Maleki, Behzad Baradaran, Jalal Abdolalizadeh, Fatemeh Ezzatifar & Jafar Majidi
Purpose: Monoclonal antibodies and related conjugates are key reagents used in biomedical researches as well as, in treatment, purification and diagnosis of infectious and non- infectious diseases. Methods: Balb/c mice were immunized with purified human IgG. Spleen cells of the most immune mouse were fused with SP2/0 in the presence of Poly Ethylene Glycol (PEG). Supernatant of hybridoma cells was screened for detection of antibody by ELISA. Then, the sample was assessed for cross-reactivity with...