Thin Layer Chromatographic Analysis of Beta-Lactam Antibiotics

Gabriel Hancu, Brigitta Simon, Hajnal Kelemen, Aura Rusu, Eleonora Mircia & Árpád Gyéresi
Purpose: The paper describes some thin layer chromatographic procedures that allow simple and rapid separation and identification of penicillins and cephalosporins from complex mixtures. Methods: Using silicagel GF254 as stationary phase and selecting different mobile phases we succeeded in the separation of the studied beta-lactamins. Our aim was not only to develop a simple, rapid and efficient method for their separation but also the optimization of the analytical conditions. Results: No system will separate all...