Supporting data: 3D projection electrophoresis for single-cell immunoblotting (Part 4)

Samantha Grist, Amy Herr & Andoni Mourdoukoutas
Immunoassays and mass spectrometry are powerful single-cell protein analysis tools; however, interfacing and throughput bottlenecks remain. Here, we introduce three-dimensional single-cell immunoblots to detect both cytosolic and nuclear proteins. The 3D microfluidic device is a photoactive polyacrylamide gel with a microwell array-patterned face (x-y) for cell isolation and lysis. Single-cell lysate in each microwell is ‘electrophoretically projected’ into the 3rd dimension (z-axis), separated by size, and photo-captured in the gel for immunoprobing and confocal/light-sheet imaging....
3 citations reported since publication in 2020.
24 views reported since publication in 2020.

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