Longitudinal analysis of the antibody repertoire of a Zika virus-infected patient revealed dynamic changes in antibody response

Xuefeng Niu, Qihong Yan, Zhipeng Yao, Fan Zhang, Linbing Qu, Chunlin Wang, Chengrui Wang, Hui Lei, Chaoming Chen, Renshan Liang, Jia Luo, Qian Wang, Lingzhai Zhao, Yudi Zhang, Kun Luo, Longyu Wang, Hongkai Wu, Tingting Liu, Pingchao Li, Zhiqiang Zheng, Yee Joo Tan, Liqiang Feng, Zhenhai Zhang, Jian Han, Fuchun Zhang … & Ling Chen
The Zika virus (ZIKV) is a mosquito-borne flavivirus that causes neonatal abnormalities and other disorders. Antibodies to the ZIKV envelope (E) protein can block infection. In this study, next-generation sequencing (NGS) of immunoglobulin heavy chain (IgH) mRNA transcripts was combined with single-cell PCR cloning of E-binding monoclonal antibodies for analysing antibody response in a patient from the early stages of infection to more than one year after the clearance of the virus. The patient's IgH...
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