Robust neutralization assay based on SARS-CoV-2 S-protein-bearing vesicular stomatitis virus (VSV) pseudovirus and ACE2-overexpressing BHK21 cells
Hua-Long Xiong, Yang-Tao Wu, Jia-Li Cao, Ren Yang, Ying-Xia Liu, Jian Ma, Xiao-Yang Qiao, Xiang-Yang Yao, Bao-Hui Zhang, Ya-Li Zhang, Wang-Heng Hou, Yang Shi, Jing-Jing Xu, Liang Zhang, Shao-Juan Wang, Bao-Rong Fu, Ting Yang, Sheng-Xiang Ge, Jun Zhang, Quan Yuan, Bao-Ying Huang, Zhi-Yong Li, Tian-Ying Zhang & Ning-Shao Xia
The global pandemic of coronavirus disease 2019 (COVID-19) is a disaster for human society. A convenient and reliable neutralization assay is very important for the development of vaccines and novel drugs. In this study, a G protein-deficient vesicular stomatitis virus (VSVdG) bearing a truncated spike protein (S with C-terminal 18 amino acid truncation) was compared to that bearing the full-length spike protein of SARS-CoV-2 and showed much higher efficiency. A neutralization assay was established based...
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