Fluorescence microscopy B cell data set

Veit Wiesmann
Fluorescence microscopy data set of B cells with corresponding ground truth. The images were captured with an Zeiss AxioSscan.Z1.
Sample Preparation:Naive murine B cells from C57Bl/6 mice were isolated from the spleen by negative selection. The cells
were activated with lipopolysaccharide (LPS, 10 µg/ml) for 72 h in RPMI1640 medium supplemented with
fetal calf serum (FCS)(10%), L-glutamine (2 mM), Pyruvate (1 mM), Penicillin (50 U/ml), Streptomycin
(50 µg/ml) and ?-Mercaptoethanol (50 µM). Teflon-coated microscope slides with 8 wells each...
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