Knockdown of miR-1275 protects against cardiomyocytes injury through promoting neuromedin U type 1 receptor

Zhu Zeng, Haixin Ma, Jing Chen, Nina Huang, Yudan Zhang, Yufei Su & Huifang Zhang
The present study aimed to assess the role of miR-1275 in cardiac ischemia reperfusion injury. H9 human embryonic stem cell (hESC)-derived cardiomyocytes stimulated by oxygen-glucose deprivation/reoxygenation (OGD/R) were used to simulate myocardial injury in vitro. miR-1275 expression levels in cells were measured by RT-qPCR. The release of lactate dehydrogenase (LDH) and creatine kinase (CK) was examined through LDH and CK ELISA kits. Cell apoptosis was detected through flow cytometry. A Fura-2 Calcium Flux Assay Kit...
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