Characterizing N-terminal variants of iCre-ERT2 in the context of a T2A ribosome skip sequence.
Two recent papers, Kats et al., Molecular Cell, 70, 488-501 (2018) and Reinhardt et al., Stem Cell Reports, 14, 1-8 (2020), suggest N-terminal amino acids are important for protein stability (in addition to the previous work in the literature).
This might be a way to modulate the abundance of the second protein. For some proteins, such as the iCre-ERT2 recombinase, that could be...