Establishing functional lentiviral vector production in a stirred bioreactor for CAR-T cell therapy

Qu-Lai Tang, Li-Xing Gu, Yao Xu, Xing-Hua Liao, Yong Zhou & Tong-Cun Zhang
As gene delivery tools, lentiviral vectors (LV) have broad applications in chimeric antigen receptor therapy (CAR-T). Large-scale production of functional LV is limited by the adherent, serum-dependent nature of HEK293T cells used in the manufacturing. HEK293T adherent cells were adapted to suspension cells in a serum-free medium to establish large-scale processes for functional LV production in a stirred bioreactor without micro-carriers. The results showed that 293 T suspension was successfully cultivated in F media (293...
1 citation reported since publication in 2021.
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