Role and function of the Hintw in early sex differentiation in chicken (Gallus gallus) embryo

Changhua Sun, Kai Jin, Jing Zhou, Qisheng Zuo, Jiuzhou Song, Zhang Yani, Guohong Chen & Bichun Li
Mono-Sex culturing is an important methodology for intensive livestock and poultry production. Here, Hintw was identified as a potential key gene in sex-determination process in chickens via RNA-seq. Then we developed an effective method to interfere or overexpress Hintw in chicken embryos through the intravascular injection. QRT-PCR, ELISA and H&E staining were used to detect the effects of Hintw on gonadal development of chicken embryos. Results showed that Hintw exhibited a female-biased expression pattern in...
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