Hsa-miR-186-3p suppresses colon cancer progression by inhibiting KRT18/MAPK signaling pathway

Tian Xia, Zhiyong Zhang, Xin Zhang & Quanfu Li
This study aimed to determine the effect of miR-186-3p and KRT18 interaction on the biological behavior of colon cancer cells. A biotin-microRNA pull-down assay was performed to identify potential miRNAs. qRT-PCR was used to verify the KRT18 and miR-186-3p levels. In addition, Western blotting was used to detect the KRT18 protein levels. The functional connection between KRT18 and miR-186-3p was confirmed using a dual luciferase reporter assay. BrdU incorporation, MTT assay, and flow cytometry were...
1 citation reported since publication in 2022.
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