The litchi semen are traditional medications for treating liver fibrosis (LF) in China. The mechanism remains unclear. This study investigates the anti-liver fibrotic mechanism of the total flavonoids of litchi semen (TFL). Sprague-Dawley rats with carbon tetrachloride-induced LF were treated with TFL (50 and 100 mg/kg) for 4 weeks. The anti-liver fibrotic effects of TFL were evaluated and the underlying mechanisms were investigated via histopathological analysis, proteomic analysis and molecular biology technology. Significant anti-LF effects...