Construction of a membrane yeast two-hybrid library and its application in identification of PsARRO-1-interacting proteins in tree peony

Huiqin Wang, Zheng Wang, Xueyuan Lou & Songlin He
To investigate the molecular mechanism of the ADVENTITIOUS ROOTING RELATED OXYGENASE 1 (ARRO-1) gene in adventitious rooting of tree peony, the PsARRO-1 gene was isolated from Paeonia ostii. Subsequently, a yeast two-hybrid (Y2H) cDNA library using the split-ubiquitin yeast membrane system was constructed, the insert fragments lengths of pPR3-N membrane Y2H library were mainly concentrated between 500 and 3,000 bp, and the library capacity reached 1.3 × 107 CFUs, with an efficiency of recombinant of...
1 citation reported since publication in 2022.
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