Investigating the role of RNA structures in transcriptional pausing using in vitro assays and in silico analyses

Simon Jeanneau, Pierre-Étienne Jacques & Daniel A. Lafontaine
Transcriptional pausing occurs across the bacterial genome but the importance of this mechanism is still poorly understood. Only few pauses were observed during the previous decades, leaving an important gap in understanding transcription mechanisms. Using the well-known Escherichia coli hisL and trpL pause sites as models, we describe here the relation of pause sites with upstream RNA structures suspected to stabilize pausing. We find that the transcription factor NusA influences the pause half-life at leuL,...
1 citation reported since publication in 2022.
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