Depleting energy sources result in a growing interest in the conversion of microalgal biofuel to improve economic feasibility. Efficient and cost effective biomass conversion to biofuel demands an increase of carbohydrates and lipid accumulation in algal cells. In the present study, it is measured that concomitant nitrogen, phosphorous and, iron deficiency induces significant physiological and biochemical changes in microalgae Oedogonium sp., Ulothrix sp., Cladophora sp. and Spirogyra sp. by using Response surface methodology as the...