Additional file 6 of miR-146a-5p-modified hUCMSC-derived exosomes facilitate spinal cord function recovery by targeting neurotoxic astrocytes
Xunwei Lai, Yang Wang, Xiaokang wang, Bin Liu & Limin Rong
Additional file 6. Identification of hUCMSC, primary astrocyte and hUCMSC-derived exosomes. A hUCMSCs were identified by the stem cell makers (CD73, CD90 and CD105) and nonstem cell markers (CD45, CD34 and HLA-DR) expression using flow cytometry. B Primary astrocytes were characterized by immunostaining of GFAP, S100β, Vimentin and morphology in bright filed. C The morphology of exosomes detected by transmission electron microscopy. Scale bar = 200 nm. D Diameters and concentrations of exosomes analyzed by...
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