Additional file 1 of High-frequency electrical stimulation attenuates neuronal release of inflammatory mediators and ameliorates neuropathic pain

Huan Yang, Timir Datta-Chaudhuri, Sam J. George, Bilal Haider, Jason Wong, Tyler D. Hepler, Ulf Andersson, Michael Brines, Kevin J. Tracey & Sangeeta S. Chavan
Additional file 1: Supplementary Figure 1. Light activated sensory neurons release inflammatory mediators. DRG sensory neurons harvested from Vglut2-Cre/ChR2-eYFP mice were cultured for 48–72 h and then stimulated with blue light (470 nm) at 20 Hz, 10% duty cycle for 15 min. Supernatant was harvested at indicated time points, and levels of HMGB1 (A), substance P (B) and CGRP (C) were quantified. N = 4-5 per group. *P < 0.05, **P < 0.01, ***P <...
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