Additional file 2: Supplementary figure 2. BNRF1 is undetectable in SF antigen-specific ACPA-expressing B cells. Left: B2M copies in DNA of SF IgG memory B cells and ACPA-expressing B cells as detected by qPCR. Right: no detection of BNRF1 by qPCR in DNA of SF IgG memory B cells, ACPA-expressing B cells and CD40L feeder cells only. Each dot represents a pool of 20 cells isolated directly into lysis buffer, from one donor. qPCR experiments...