Additional file 1 of Active human full-length CDKL5 produced in the Antarctic bacterium Pseudoalteromonas haloplanktis TAC125Andrea Colarusso, Concetta Lauro, Marzia Calvanese, Ermenegilda Parrilli & Maria Luisa Tutino
Additional file 1: Table S1. Characteristics of BCD constructs for pGFP expression. Table S2. List of primers used in this work. Fig. S1. Average plasmid copy number (PCN) of pP79-107 (B). Fig. S2. Ranking of bicistronic designs (BCDs) with a fluorescent reporter. Fig. S3. flCDKL5 production profiles with pBCD-107 (L) plasmids. Fig. S4. Development of Bicistronic Entry Clones. Fig. S5. Development of Bicistronic Designs. Fig. S6. Development of Tricistronic Designs.
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