Additional file 3: Fig. S3. Verification of 5-HT2B knockout flies. (A) Molecular verification of 5-HT2B knockout flies by PCR analysis. Only one band was seen in w1118 controls, but a lower band could exist when a fragment of approximately 233 bp was deleted from the 5-HT2B locus. (B) The PCR-Sanger sequencing results of two reserved homozygous 5-HT2B knockout lines. The results show that the 5-HT2BKO_5-1 line has a 224 bp deletion in the first exon...