Tissue RNA was isolated from fresh mouse colon sample by Trizol reagent. Total RNA (1 µg) was reverse-transcribed using a reverse transcriptase kit (TakaRa, cat. RR036A-1, Tokyo, Japan). Hieff® qPCR SYBR Green Master Mix (Yeasen Biotechnolog, cat. 11201ES03, Shanghai, China) was used for Real-time qPCR. The comparative cycle threshold (Ct) method (2−ΔΔCT) was used to calculate relative expression, β-actin was used as the internal reference. We evaluated immunostaining by multiplying the intensity score and proportion...