3D Ecoli nucleoid stained with SYBR-Gold

Simona Streckaite, Dmitrij Frolov, Jevgenij Chmeliov, Andrius Gelzinis, Cristian Ilioaia, Sylvie Rimsky, Rienk van Grondelle, Leonas Valkunas, Andrew Gall & Bruno Robert
Movie S1 shows volumetric 3D reconstruction of the E. coli bacterial chromatin stained with SYBR® Gold—a rotation around y axis. The sample was scanned with 40 nm x & y steps, and 200 nm z steps, λexc = 488 nm, emission collected at 505–545 nm window. 3D reconstruction was obtained from 12 planes.
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