Additional file 1 of Functional analysis of the dehydratase domains of the PUFA synthase from Emiliania huxleyi in Escherichia coli and Arabidopsis thaliana
Bihan Chen, Feng Wang, Xi Xie, Huifan Liu, Dongjie Liu, Lukai Ma, Gengsheng Xiao & Qin Wang
Additional file 1: Table S1. Production of SFAs and UFAs in wild type Escherichia coli Rosetta overexpressing EhDH domains. Table S2. Production of SFAs and UFAs in wild-type Escherichia coli Rosetta overexpressing the mutated EhDH domains. Table S3. Primers used for DH domain amplifications. Table S4. Primers used for site-directed mutagenesis of DH domains. Table S5. Primers used for plant experiment. Figure S1. Expression of recombinant proteins when induced with 0.2 mM IPTG in Escherichia...
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