Additional file 2 of Sphingosine kinase 1 contributes to the metastatic potential of epithelial ovarian cancer to the adipocyte-rich niche

Chen Wang, Taiyang Ye, Wenjing Wang, Keqi Song, Jie Zhu, Lan Dai & Wen Di
Additional file 2: Fig. S1 Inhibition of SphK1 by siRNA suppressed adipocyte-induced metastasis of HEY cells. A Adipocyte isolation. Primary human omentum tissues were collected and mature adipocytes were extracted. Adipocytes were visualized by (i) microscopy, (ii) stained with Oil red O to confirm the extraction of mature adipocytes, and (iii) by fluorescence microscopy with calcein-AM to confirm viability. The scale bar represents 100 μm. B HEY cells were transfected with negative control siRNA or...
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