Additional file 2 of Opposing effects of apoE2 and apoE4 on microglial activation and lipid metabolism in response to demyelination

Na Wang, Minghui Wang, Suren Jeevaratnam, Cassandra Rosenberg, Tadafumi C. Ikezu, Francis Shue, Sydney V. Doss, Alla Alnobani, Yuka A. Martens, Melissa Wren, Yan W. Asmann, Bin Zhang, Guojun Bu & Chia-Chen Liu
Additional file 2: Figure sup 2. Lack of effects of cuprizone-induceddemyelination on the blood-brainbarrier integrity among apoE-TR mice. (A) Brain samples ofapoE-TR mice from CPZ-treated group (n=16/genotype) were subjected toimmunofluorescence staining for CLDN5(a tight junction-associated protein) and Glut1 (vascular marker). Representative images of CLDN5 (Green) staining and Glut1 (Red)staining in the CC region of apoE-TR mice are shown. Scale bar, 50 µm. Values are mean ± SEM. One-way ANOVA.N.S. not significant.
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