Generation of self-replicating airway organoids from the cave nectar bat Eonycteris spelaea as a model system for studying host–pathogen interactions in the bat airway epithelium

Louisa L.Y. Chan, Akshamal M. Gamage, Chee Wah Tan, Kai Sen Tan, Jing Liu, Douglas Jie Wen Tay, Randy Jee Hiang Foo, Laurent Rénia, De Yun Wang & Lin-Fa Wang
Bats are reservoir hosts for various zoonotic viruses with pandemdic potential in humans and livestock. In vitro systems for studying bat host–pathogen interactions are of significant interest. Here, we establish protocols to generate bat airway organoids (AOs) and airway epithelial cells differentiated at the air–liquid interface (ALI-AECs) from tracheal tissues of the cave-nectar bat Eonycteris spelaea. In particular, we describe steps which enable laboratories that do not have access to live bats to perform extended...
This data repository is not currently reporting usage information. For information on how your repository can submit usage information, please see our documentation.