Substrates for helicase assays generated by PCR

Jae-Ho Shin, John N. Reeve & Zvi Kelman
Copyright information:Taken from "Use of a restriction enzyme-digested PCR product as substrate for helicase assays"Nucleic Acids Research 2005;33(1):e8-e8.Published online 13 Jan 2005PMCID:PMC546180.© 2005, the authors © A PCR product and its restriction fragment derivatives are shown. PCR reactions were performed as described in Materials and Methods with P-labeled primer, and products were purified using the QIAquick PCR purification kit (Qiagen) (lane 1) and digest with PstI (lane 2), EcoRI (lane 3) and SmaI (lane 4)...
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