Gliding motility assay using 22063mM D2O in solution. Replicate 1/3. ROI 4/15.

Andy Maloney
• This is a gliding motility assay using kinesin-1 and rhodamine labeled microtubules. • The buffer used was PEM (also known as BRB80) with the inclusion of 22063mM D2O. • Images were taken with an Andor Luca EMCCD camera on an Olympus IX-71 microscope using a 60x 1.42NA PlanApo objective. • This fileset is replicate 1/3 and ROI 4/15. • Images were taken to investigate speed variations of microtubules due to the inclusion of D2O...
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