Three dimensional super-resolved live cell imaging through polarized multi-angle TIRF

Cuifang Kuang, Cheng Zheng, Guangyuan Zhao, Wenjie Liu, Youhua Chen, Zhimin Zhang, Luhong Jin, Xu Liu & Yingke Xu
Measuring three dimension nanoscale cellular structures is challenging, especially when the structure is dynamic. Owing to the informative total internal reflection fluorescence (TIRF) imaging under varied illumination angles, multi-angle (MA) TIRF has been examined to offer a nanoscale axial and a sub-second temporal resolution. However, conventional MA-TIRF still performs badly in lateral resolution and fails to characterize the depth-image in densely-distributed regions. Here, we for the first time, emphasize the lateral super-resolution in the MA-TIRF...
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