A simplified multiplex methylated DNA testing for early detection of colorectal cancer in stool DNA

Yanmiao Dai, Guodong Zhao, Jun Yang, Xilang Zhou, Shangmin Xiong, Xirong Lu, Liming Gao, Jianfang Wu, Zouhua Xu, Sujuan Fei, Minxue Zheng & Hongwei Xu
Abstract Background ColoDefense1.0 assay has demonstrated its excellent sensitivity and specificity for early detection of colorectal cancer (CRC) by detecting the methylation levels of SDC2 and SEPT9, while exhibited limitations on relatively large sample capacity required and limited detection throughput by applying triplicate PCR reactions for each sample. In this study, ColoDefense1.0 was simplified and optimized into ColoDefense2.0 in a single PCR reaction. Methods A total 529 stool specimens were collected, and 244 CRC patients,...
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