Modification and application of highly active alkaline pectin lyase

Pi-Wu Li, Jun Ma, Xiao-Feng Wei, Zi-Yang Zhang, Rui-Ming Wang, Jing Xiao & Jun-Qing Wang
Abstract Alkaline pectate lyase has developmental prospects in the textile, pulp, paper, and food industries. In this study, we selected BacPelA, the pectin lyase with the highest expression activity from Bacillus clausii, modified and expressed in Escherichia coli BL21(DE3). Through fragment replacement, the catalytic activity of the enzyme was significantly improved. The optimum pH and temperature of the modified pectin lyase (PGLA-rep4) were 11.0 and 70 °C, respectively. It also exhibited a superior ability to...
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