Extracellular vesicles from oviductal and uterine fluids supplementation in sequential in vitro culture improves bovine embryo quality

Cláudia Lima Verde Leal, Karina Cañón-Beltrán, Yulia N. Cajas, Meriem Hamdi, Aracelli Yaryes, María Gemma Millán de la Blanca, Paula Beltrán-Breña, Rosane Mazzarella, Juliano Coelho da Silveira, Alfonso Gutiérrez-Adán, Encina M González & Dimitrios Rizos
Abstract Background In vitro production of bovine embryos is a well-established technology, but the in vitro culture (IVC) system still warrants improvements, especially regarding embryo quality. This study aimed to evaluate the effect of extracellular vesicles (EVs) isolated from oviductal (OF) and uterine fluid (UF) in sequential IVC on the development and quality of bovine embryos. Zygotes were cultured in SOF supplemented with either BSA or EVs-depleted fetal calf serum (dFCS) in the presence (BSA-EV...
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